Bromodeoxyuridine labeling for S-phase measurement in breast carcinoma
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منابع مشابه
Double Labeling of S-Phase Murine Cells with Bromodeoxyuridine and a Second DMA-specific Probe1
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in Sphase was brightly fluorescent (RPMB te...
متن کاملDouble labeling of S-phase murine cells with bromodeoxyuridine and a second DNA-specific probe.
A rapid method has been developed which combines immunofluorescence and autoradiography and permits the double labeling of DNA. P388 murine leukemic cells were incubated with bromodeoxyuridine and tritiated thymidine simultaneously. After fixation, the sample was first processed with a monoclonal antibody to bromodeoxyuridine (RPMB I) so that any cell in S-phase was brightly fluorescent (RPMB t...
متن کاملComparison of bromodeoxyuridine and proliferating cell nuclear antigen labeling in gastric carcinoma.
The proliferative activity of human gastric carcinoma was measured by means of in vitro incorporation of the thymidine analogue, 5-bromo-2'-deoxyuridine (BrdU), into the newly-synthesized DNA of fresh tumors and immunohistochemical staining of proliferating cell nuclear antigen (PCNA) using avidin-biotin peroxidase method. Eighty-two cases of surgically resected human gastric carcinomas consist...
متن کاملIn vitro immunohistochemical localization of S-phase cells by a monoclonal antibody to bromodeoxyuridine.
Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In vitro immunohistochemical application of the BrdU/anti-BrdU-MAb method permits a quantitative assessment of the proliferative activity of a tissue as well as the direct location of the actively replicating cells in histological sections. In...
متن کاملTyrosine aminotransferase sensitivity to bromodeoxyuridine during restricted intervals of S phase in hepatoma cells
Synchronized hepatoma tissue culture (HTC) cells, accumulated at the G1/S boundary with aminopterin, were released into S phase with either thymidine or 5-bromodeoxyuridine (BUdR). Tyrosine aminotransferase (TAT) activity was found to be unaffected by BUdR over the initial 3 h of S phase, but then to rapidly decline to a new basal level of 40% of control by 9 h. There was no corresponding respo...
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ژورنال
عنوان ژورنال: Cancer
سال: 1993
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(19930601)71:11<3531::aid-cncr2820711112>3.0.co;2-0